NICHOLAS G. CRAWFORD Email: ngcrawford@gmail.com Website: ngcrawford.com Twitter: twitter.com/ngcrawford Github: github.com/ngcrawford AREAS OF SPECIALIZATION Bioinformatics, evolutionary biology, computational biology, population genomics, phylogenomics, speciation, ultra conserved elements, herpetology and entomology EDUCATION 2013, Postdoctoral Fellow, California Academy of Sciences Advisors: Durrell Kapan and Brian Simison 2007/13, Ph.D. Biology, Boston University Advisor: Christopher Schneider Thesis Title: Anolis Lizard Genomics and Phylogenetics Additional Projects: Mammal, Reptile, and Avian Phylogenomics, with Travis Glenn Butterfly Speciation Genomics, with Sean Mullen 2004/07, M.S. Biology, San Diego State University Advisor: Tod Reeder Thesis: Population structure within the Plateau Striped Whiptail (Aspidoscelis velox complex) a parthenogenetic lizard Additional Project: Phylogenetic relationships among Australian skinks of the genus Glaphyromorphus 1997/01, B.S. Biology, Union College Graduated Cum Laude with Academic and Departmental Honors SKILLS AND TECHNIQUES Programming Languages/Frameworks: Python (Modules: Numpy, Scipy, Matplotlib, Biopython, Pysam, Multiprocessing, iPython, MrJob, Pandas, Flask, and Django), MySQL/Sqlite3, Git/Github, Unix/Linux, LSF/SGE/AWS Clusters, Hadoop/MapReduce, also some R, Perl, Html, and CSS Software: GATK, ABySS, Trinity, Breakdancer, Samtools, VCFtools, Tophat, Cufflinks, Bowtie2, Stampy, GO Elite, Blast/NCBI, MrBayes, Sequencher, Oligo (primer design), Genemapper, GenAlEx, Arlequin Laboratory: Genomic, RAD-tag, and RNA-seq Illumina library construction, Primer Design, DNA purification, PCR, qPCR (Taqman and Kappa assays), Sanger Sequencing (both ABI3130 and ABI377 genetic analyzers), Microsatellite Genotyping, Bioanalyzer, and constructing cDNA libraries Miscellaneous Skills: Protein Purification (FPLC), Mammalian Tissue Culture, Ordering and Stocking, Maintaining Laboratory Compliance with Safety Regulations, Training Students and Techs, and Catching lizards GRANTS AND SCHOLARSHIPS 2012 - Grand Challenges Award, Smithsonian Institute Consortia, Next Generation Phylogenetics, MJ Braun, K Wurdack, W Wcislo, J Maldonado, K Helgen, S Brady, M Cummings, TC Glenn, BC Faircloth, RT Brumeld, E Braun, JC McCormack, NG Crawford, N White. $100,000. 2011 - Next-generation Sequencing Small Grant. Phylogenetic utility of ultra- conserved elements for the avian tree of life. MJ Braun, ND White, TC Glenn, BC Faircloth, RT Brumeld, EL Braun, JE McCormack, NG Crawford. $10,000 2011 - Amazon Education Research Grant (aws.amazon.com), Computational Resources. BC Faircloth, NG Crawford, JE McCormack. $10,000. 2010 - Amazon Education Research Grant (aws.amazon.com), Computational Resources. NG Crawford, BC Faircloth, TC Glenn. $7,500. 2010 - Doctoral Dissertation Improvement Grant (NSF). NG Crawford, C Schneider. $15,000. 2009 - Theodore Roosevelt Memorial Fund (American Museum of Natural History) labwork/fieldwork. $1,500. 2005 - Theodore Roosevelt Memorial Fund (American Museum of Natural History) labwork/fieldwork. $1,960. 2005 - Harry E. Hamber Memorial Scholarship, tuition. $1,400. 2000 - IEF Grant, thesis research. $100. 2000 - NYSEP Grant, summer stipend. $1,700. 1999 - Booth - Ferris Grant, summer stipend. $1,700. PUBLICATIONS Marcus R. Kronforst, Matthew E. B. Hansen, Nicholas G. Crawford , Jason R. Gallant, Rob J. Kulathinal, Durrell D. Kapan, Sean P. Mullen. 2013. Hybridization reveals the evolving genomic architecture of speciation. Cell Reports, 5, 1–12. John E. McCormack, Michael G. Harvey, Brant C. Faircloth, Nicholas G. Crawford, Travis C. Glenn, Robb T. Brumfield. 2013. A phylogeny of birds based on over 1,500 loci collected by target enrichment and high-throughput sequencing. PLoS ONE. 8(1): e54848. Martha M. Muñoz, Nicholas G. Crawford, Thomas J. McGreevy, Rebecca D. Tarvin, Nicholas J. Messana, Liam J. Revell, Rosanne M. Zandvliet, Juanita M. Hopwood, Elbert Mock, André L. Schneider, and Christopher J. Schneider. 2013. Divergence in coloration and the evolution of reproductive isolation in the Anolis marmoratus species complex. Molecular Ecology. 22(10), 2668–2682. John E. McCormack, Michael G. Harvey, Brant C. Faircloth, Nicholas G. Crawford, Travis C. Glenn, Robb T. Brumfield. 2013. A phylogeny of birds based on over 1,500 loci collected by target enrichment and high-throughput sequencing. PLoS ONE. 8(1), e54848. John A. St. John, Edward L. Braun, Sally R. Isberg, Lee G. Miles, Amanda Y. Chong, Jamie Gongora, Pauline Dalzell, Christopher Moran, Taisen Iguchi, Bertrand Bed'Hom, Shane C. Burgess, Amanda M. Cooksey, Todd A. Castoe, Arkhat Abzhanov, Llewellyn D. Densmore, Miryam Venegas-Anya, Matthew J. Greenwold, Roger H. Sawyer, Federico G. Hoffmann, Nicholas G. Crawford, Jennifer C. Drew, Scott V. Edwards, Matthew K. Fujita, Jonathan M. Howard, Brant C. Faircloth, Daniel E. Janes, Shahid Yar Khan, Satomi Kohno, A.P. Jason de Koning, Stacey L. Lance, Fiona M. McCarthy, John E. McCormack, Mark E. Merchant, Daniel G. Peterson, David D. Pollock, Nader Pourmand, Brian J. Raney, Kyria A. Roessler, Jeremy R. Sanford, Carl J. Schmidt, Eric W. Triplett, Tracey D. Tuberville, Erich D. Jarvis, Louis J. Guillette Jr, Travis C. Glenn, Richard E. Green and David A. Ray. 2012. Sequencing three crocodilian genomes to illuminate the evolution of archosaurs and amniotes. Genome Biology. 13, 415. Kenro Kusumi, Rob J. Kulathinal, Arhat Abzhanov, Stephane Boissinot, Nicholas G. Crawford, Brant C. Faircloth, Travis C. Glenn, Daniel E. Janes, Jonathan B. Losos, Douglas B. Menke, Steven Poe, Thomas J. Sanger, Christopher J. Schneider, Jessica Stapley, Juli Wade, Jeanne Wilson-Rawls. 2012. Developing a community-based genetic nomenclature for anole lizards. BMC genomics, 12(1), 554. Nicholas G. Crawford, Brant C. Faircloth, John E. McCormack, Robb T. Brumfield, Kevin Winker, Travis C. Glenn. 2012. More than 1000 ultraconserved elements provide evidence that turtles are the sister group of archosaurs. Biology Letters. 8(5), 783-786. Brant C. Faircloth, John E. McCormack, Nicholas G. Crawford, Michael Harvey, Robb T. Brumfield, Travis C. Glenn. 2012. Ultraconserved elements anchor thousands of genetic markers for target enrichment spanning multiple evolutionary timescales. Systematic Biology. 61(5), 713-715. John E. McCormack, Brant C. Faircloth, Nicholas G. Crawford, Patricia Adair Gowaty, Robb T. Brumfield, Travis C. Glenn. 2011. Ultraconserved Elements Are Novel Phylogenomic Markers that Resolve Placental Mammal Phylogeny when Combined with Species Tree Analysis. Genome Research. 22(4), 746-54. Nicholas G. Crawford. 2010. SMOGD: Software for the Measurement of Genetic Diversity. Molecular Ecology Resources, 10: 556-557. Nicholas G. Crawford, Jaime Zaldvar-Rae, Cris Hagen, Amanda Schable, Erica Bree Rosenblum, Jeff A. Graves, Tod W. Reeder, Michael G. Ritchie, Travis C. Glenn. 2007. Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards. Molecular Ecology Resources. 8: 219-223 Nicholas G. Crawford, Cris Hagen, Heather F. Sahli, Elizabeth A. Stacy, Travis C. Glenn. 2007. Fifteen polymorphic microsatellite loci from Hawaiis Metrosideros polymorpha Myrtaceae: Myrtales), a model species for ecology and evolution. Molecular Ecology Resources, 8, 308-310. Caleb R. Hickman, Maureen B. Peters, Nicholas G. Crawford, Cris Hagen, Travis C. Glenn, Christopher M. Sommers. 2008. Development and characterization of microsatellite loci in the American white pelican (Pelecanus erythrorhynchos). Molecular Ecology Resources, 8, 1439-1441. Nicholas G. Crawford, Maureen B. Peters, Cris Hagen, Travis C. Glenn, Stephen K. Davis, Christopher M. Somers. 2007. Twelve polymorphic microsatellite loci from Spragues pipit, Anthus spragueii (Motacillidae:Passeriformes), a threatened grassland endemic songbird. Molecular Ecology Resources, 9, 315-317. Nicholas G. Crawford. 2007. Microsatellites in cnemidophorine lizards: their utility in investigating the landscape genetics of the plateau striped whiptail (Aspidoscelis velox Complex). Masters Thesis: San Diego State University. Olga V. Tsyusko, Tracey D. Tuberville, Maureen B. Peters, Nicholas G. Crawford, Cris Hagen, Steve Weller, Ann Sakai, and Travis C. Glenn. 2007. Microsatellite markers isolated from polyploid woodsorrell (Oxalis alpina). Molecular Ecology Notes, 7, 1284-1286. PROFESSIONAL EXPERIENCE 2007-2011 Teaching Fellow, Boston University: Introductory Biology, Genetics, Evolution, Animal Behavior 2007 - South Carolina, Summer, 6 months, Research Technician, supervised by Travis Glenn 2006 - South Carolina, Summer, 6 weeks, Microsatellite loci preparation at the Savannah River Ecology Laboratory, supervised by Travis Glenn 2004/2005 - Graduate Teaching Assistant, SDSU, Introductory Biology and Introductory Zoology 2004 - Research Assistant, SDSU, Advisor: Tod Reeder 2002/03 - Research Associate, Boston Biochem 2001 - Research Associate, Pfizer Pharmaceuticals 2001 - Research Associate, Harvard Medical School FIELD EXPERIENCE 2012 - Guantanamo Bay, Cuba, Summer, 1 week, collecting lizards 2009 - Guadeloupe, French West Indies, Summer, 5 days, collecting lizards 2007 - New Mexico, Summer, 1 week, collecting lizards 2005 - New Mexico, Summer, 1 week, collecting lizards 2005 - Arizona, Summer, 2 weeks, collecting lizards 2004 - Arizona, Summer, 2 weeks, collecting lizards 2001 - New York, Summer, 1 week, collecting treefrogs 1999 - New York, Summer, 1 week, collecting treefrogs CONFERENCES, MEETINGS, AND TALKS 2013 Joint annual meeting of the Society for the Study of Evolution and the Society of Systematic Biologists Presentation Title: Genomics of local adaptation and colorful pigmentation in Anolis lizards 2013 - UMass Lowell Seminar Series: Invited Speaker Presentation Title: From Archosaurs to Anoles: Genomic Approaches to Studying Reptile Evolution 2012 - Union College Seminar Series: Invited Speaker - Union College. Presentation Title: Genomic approaches to understanding reptile evolution. 2012 - World Congress of Herpetology, Vancouver. Presentation Title: The Genetics of Colorful Pigmentation in Anolis Lizards. 2012 - Evolutionary Genomics Super Group, Broad Institute Presentation Title: Ultraconserved Elements as Phylogenomic Markers. 2012 - MCZ Lunchtime Seminar, Harvard University Presentation Title: Thousands of ultraconserved elements in combination with cloud computing and species-tree methods help resolve deep divergences in reptiles, mammals, and birds 2010 - The Genetics and Evolution of Animal Coloration, Radcliffe Workshop Presentation Title: Identifying coloration genes when you can’t easily do QTL mapping 2009 - Anole Symposium Harvard University Presentation Title: Anolis carolinensis: Pigmentation Genetics Poster Title: Genome scan identifies two loci associated with color polymorphism in Anolis marmoratus 2009 - Gordon Conference: Evolutionary & Ecological Functional Genomics - Tilton, New Hampshire. Poster Title: Genetics of Colorful Pigmentation in Anolis Lizards 2009 - Society for Integrative and Comparative Biology Annual Meeting, Boston Massachusetts. Presentation Title: Evolution of Dewlap Pigmentation in Anoline lizards 2008 - Union College Seminar Series: Invited Speaker - Union College Presentation Title: Population Structure of the Plateau Striped Whiptail a Parthenogenetic Species of Lizard 2007 - Island Biogeography Symposium, Harvard University, Cambridge Massachusetts 2005 - American Society of Ichthyologists and Herpetologists 85th Annual Meeting, Tampa, Florida Presentation Title: Phylogenetic relationships among Australian skinks of the genus Glaphyromorphus 2004 - American Society of Ichthyologists and Herpetologists 84th Annual Meeting, Norman, Oklahoma 2004 - Evolution Conference, Fort Collins, Colorado SOCIETY AFFILIATIONS American Society of Ichthyologists and Herpetologists (not presently affiliated) Society for Comparative and Integrative Biology (not presently affiliated)